Anti-Cytokeratin, Basic (Type II or HMW) Antibody (SPM591) BHA10207643

Mfr.No.	MSM3X-446-P1ABX
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Clonality	Monoclonal
Description	This antibody recognizes CK1, CK5, CK10 and CK14. In normal epithelia, it stains stratified epithelia, myoepithelial cells and basal cells in the prostate gland and bronchi. This MAb shows no reactivity with hepatocytes, pancreatic acinar cells, proximal renal tubules, or endometrial glands; there is no reactivity with cells derived from simple epithelia. Mesenchymal tumors, lymphomas, melanomas, neural tumors, and neuroendocrine tumors are negative with this antibody. It stains myoepithelial cells and has been shown to be useful in distinguishing prostate adenocarcinoma from benign prostate. This antibody has also been useful in separating benign from malignant intraductal breast proliferations.
Host	Mouse
Immunogen	Solubilized keratin extract from human stratum corneumUnigeneMultiple
Positive Control	PC12 cells, Skin, Prostate carcinoma
Reactivity	Human, Mouse, Rat
Recombinant	FALSE
Regulatory	RUO

Swissprot	P04264; P13647; P13645; P02533
Uniprot	80828; 433845; 99936; 6545380
Gene Id	3848;3852;3858;3861
Clone No	SPM591
Symbol	Multiple
Weight	67kDa (CK1); 58kDa (CK5); 56.5kDa (CK10); 50kDa (CK14)

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	0.5-1.0μg/ml
WB	0.5-1.0μg/ml
IHC-F	0.25-0.5μg/ml for 30 min at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10207643	MSM3X-446-P1ABX	100µg	$393.20		Add to Cart

Anti-Cytokeratin, Basic (Type II or HMW) Antibody (SPM591) BHA10207643

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