This MAb is an excellent marker for human cells in xenographic model research. It reacts specifically with human cells. It is a part of a new panel of reagents, which recognizes subcellular organelles or compartments of human cells. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. MAb 235-1 recognizes an antigen associated with the nuclei in human cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. It can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
Host
Mouse
Immunogen
Nuclei of human myeloid leukemia biopsy cells
Positive Control
All human cells. Tonsil
Reactivity
Human, non-human primates
Recombinant
FALSE
Regulatory
RUO
Swissprot
Not Known
Uniprot
Not Known
Gene Id
Not Known
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IP, IHC(af), IHC-F
Description
Optimal dilution for a specific application should be determined., FACS ; IF ; IP ; IHC(af) ; IHC-F
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
0.5-1μg/ml
IP
0.5-1 �g/500ug protein Lysate
IHC(af)
0.25-0.5μg/ml for 30 minutes at RT
IHC-F
0.5-1.0μg/ml for 30 minutes at RT
No Description Available.
No Description Available.
No Description Available.
No Description Available.
Reviews of Anti-Human Nuclear Antigen (HNA) Antibody BHA10207636