It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
Host
Mouse
Immunogen
Biotinylated sheep immunoglobulin
Positive Control
Biotinylated proteins in solution or on tissues
Reactivity
All species
Recombinant
FALSE
Regulatory
RUO
Swissprot
Not Applicable
Uniprot
Not Applicable
Gene Id
Not Applicable
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
CM, FACS, IF, WB, IHC-F
Description
In situ Nucleic Acid Hybridization, (No special pretreatment is required for the immunohistochemical staining of formalin-fixed, paraffin-embedded tissues) Optimal dilution for a specific application should be determined., CM ; FACS ; IF ; WB ; IHC-F
Dilution
CM
FACS
0.5-1μg/million cells in 0.1ml
IF
0.5-1μg/ml
WB
IHC-F
0.5-1μg/ml for 30 minutes at RT
Reviews of Anti-Biotin (Vitamin B7 or Vitamin H) Antibody BHA10207336