Anti-Cytokeratin 8/18 Antibody BHA10207550

Mfr.No.	MSM20-1221-P1ABX
Alternative Names	CARD2 ; CK8 ; CYK8 ; CYKER ; Cytokeratin Endo A ; DreK8 ; EndoA ; K2C8 ; K8 ; Keratin 8
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Clonality	Monoclonal
Description	Cytokeratin 8 (CK8) belongs to the type II (or B or basic) subfamily of high molecular weight cytokeratins and exists in combination with cytokeratin 18 (CK18). This MAb cocktail recognizes all simple epithelia including glandular epithelium, for example thyroid, female breast, gastrointestinal tract, respiratory tract, and urogenital tract including transitional epithelium. All adenocarcinomas and most squamous carcinomas are positive but keratinizing squamous carcinomas are usually negative. This antibody is useful in demonstrating the presence of Paget cells; there is very little keratin 18 in the normal epidermis so only Paget cells are stained.ĀImmunohistochemical staining with this MAb is indistinguishable from that obtained with monoclonal antibody 5D3.
Host	Mouse
Immunogen	Cytoskeletal preparation from HeLa epitheliod carcinoma cells (B22.1) and PMC-42 breast carcinoma cells of human origin (B23.1)
Positive Control	MCF-7, HeLa, PMC-42 or A431 cells. Skin, Colon, lung or breast carcinoma
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P05787; P05783
Uniprot	533782; 708445; 406013
Gene Id	3856;3875

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	1-2μg/ml
WB	0.5-1.0μg/ml
IHC-F	0.5-1.0μg/ml for 30 min at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10207550	MSM20-1221-P1ABX	100µg	$393.20		Add to Cart

Anti-Cytokeratin 8/18 Antibody BHA10207550

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