This MAb recognizes both the free and protein-conjugated (either soluble or cell bound) form of fluorescein or carboxy-fluorescein. Sensitivity of the FITC-anti-FITC system is reportedly similar to that of the biotin-avidin system. In biotin or enzyme-labeled form, this MAb is also suitable for the conversation of fluorescein-labeled detection into immuno-enzymatic assays.
Host
Mouse
Immunogen
Fluorescein isothiocyanate (FITC) conjugated to KLH
Positive Control
Fluoresceinated proteins in solution or on tissues
Reactivity
All species
Recombinant
FALSE
Regulatory
RUO
Swissprot
Not Applicable
Uniprot
Not Applicable
Gene Id
Not Applicable
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA; 0.05% azide. Also available WITHOUT BSA; azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
CM, FACS, IF, WB, IHC-F
Description
In situ Nucleic Acid Hybridization, (Pretreatment depends upon the fluoreceinated primary antibody.)Optimal dilution for a specific application should be determined., CM ; FACS ; IF ; WB ; IHC-F
Dilution
CM
FACS
0.5-1μg/million cells
IF
0.5-1μg/ml
WB
IHC-F
0.5-1μg/ml for 30 minutes at RT
Reviews of Anti-FITC (Fluorescein Isothiocyanate) Antibody BHA10207308