This MAb recognizes cytokeratin 4, 5, 6, 8, 10, 13, and 18. This is a broad-spectrum antibody which has been reported to differentiate epithelial tumors from non-epithelial tumors. Many studies have shown the usefulness of keratins as markers in cancer research and tumor diagnosis.
Host
Mouse
Immunogen
Keratin-enriched preparation from cultured human A431
Positive Control
A431 cells, Skin, Colon carcinoma
Reactivity
Human, Cow, Rat, Mouse, Guinea pig, Frog, Goat, Marmoset, Pig
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, WB, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
0.5-1.0μg/ml
WB
0.5-1.0μg/ml
IHC-F
0.5-1.0μg/ml for 30 min at RT
Reviews of Anti-Cytokeratin, Multi Antibody (SPM583) BHA10207331