Anti-Actin, Muscle Specific Antibody BHA10207537

Mfr.No.	MSM2-953-P0
Alternative Names	ACTA ; ACTA1 ; ACTA2 ; ACTC1 ; Actin ; ACTSA ; Alpha-2 actin ; alpha skeletal muscle ; Alpha-actin-1 ; Cardiac muscle alpha actin-1
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	1q42.13 (ACTA1); 10q23.3 (ACTA2); 2p13.1 (ACTG2)
Clonality	Monoclonal
Description	This antibody recognizes actin of skeletal, cardiac, and smooth muscle cells. It is not reactive with other mesenchymal cells except for myoepithelium. Actin can be resolved on the basis of its isoelectric points into three distinctive components: alpha, beta, and gamma in order of increasing isoelectric point. Anti-muscle specific actin recognizes alpha and gamma isotypes of all muscle groups. Non-muscle cells such as vascular endothelial cells and connective tissues are non-reactive. Also, neoplastic cells of non-muscle-derived tissue such as carcinomas, melanomas, and lymphomas are negative.ĀIt stains tumors of smooth muscle (leiomyomas and leiomyosarcomas) as well as skeletal muscle (rhabdomyomas and rhabdomyosarcomas).
Host	Mouse
Immunogen	Synthetic peptide common among human muscle actins
Positive Control	Muscle or sarcoma
Reactivity	Human, Rabbit, Rat
Recombinant	FALSE
Regulatory	RUO

Swissprot	P62736; P68032; P68133
Uniprot	118127; 1288; 500483
Gene Id	58;59;70

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	200µg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml

Applications

FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution