Anti-E-Cadherin / CD324 Antibody BHA10206994

Mfr.No.	999-MSM5-P1ABX
Alternative Names	Arc 1 ; cadherin 1 type 1 E-cadherin ; Cadherin1 ; CAM 120/80 ; CD324 ; CDH1 ; CDHE ; E-Cad/CTF3 ; E-cadherin ; ECAD
Categories	Primary Antibodies
Cellular Localization	Cell Surface
Chromosome Location	16q22.1
Clonality	Monoclonal
Description	Recognizes a protein of 120-80kDa, identified as E-cadherin. Cadherins comprise a family of Ca2+-dependent adhesion molecules that function to mediate cell-cell binding critical to the maintenance of tissue structure and morphogenesis. The classical cadherins, E-, N- and P-cadherin, consist of large extracellular domains characterized by a series of five homologous NH2 terminal repeats. The relatively short intracellular domains interact with a variety of cytoplasmic proteins, such as β-catenin, to regulate cadherin function. E-cadherin plays an important role in epithelial cell adhesion. A decreased expression of E-cadherin is associated with metastatic potential and poor prognosis in breast cancer, prostate and esophageal cancer. In combination with p120 Catenin, it is useful for the differentiation between ductal (E-cadherin +) and lobular (E-cadherin -) breast carcinomas. It may also help in diagnosis of mesothelioma.
Host	Mouse
Immunogen	Recombinant human full-length E-Cadherin protein
Positive Control	LS174T, Raji, HT29, SK-BR3 cells. Prostate or Colon carcinomas.
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P12830
Uniprot	461086
Gene Id	999

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution