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Home / Primary Antibodies / Flow Cytometry (FACS)

Anti-CD53 (TSPAN25) Antibody BHA10206721

SKU : BHA10206721

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NeoBiotechnologies, Inc.

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Mfr.No.	963-MSM1-P1ABX
Alternative Names	CD53 glycoprotein ; CD53 tetraspan antigen ; Cell surface glycoprotein CD53 ; Leukocyte surface antigen CD53 ; Tetraspanin-25 (Tspan-25) ; TSPAN25
Categories	Primary Antibodies
Cellular Localization	Cell Surface
Chromosome Location	1p13
Clonality	Monoclonal
Description	Recognizes a protein of 33-55kDa, identified as CD53 (Workshop V; Code CD53.1). CD53 is expressed on monocytes, and macrophages, granulocytes, dendritic cells, osteoblasts and osteoclasts, NK cells, and on T- and B-cells from every stage of differentiation but is absent from platelets, erythrocytes, and non-haemopoietic cells. CD53 is a member of a family of tetraspan transmembrane proteins, including CD9, CD37, CD63, CD81, and CD82. It associates with integrins, MHC class II molecules, and a tyrosine phosphatase and plays a role in cellular activation as part of a signal transduction complex involving other membrane glycoproteins. Defects of CD53 expression on neutrophils appear to be related with recurrent infectious diseases. Cross-linking CD53 using CD53 antibodies led to cytoplasmic calcium fluxes in B cells, monocytes, and granulocytes and activation of the monocyte oxidative burst.
Host	Mouse
Immunogen	Human Sezary cells
Positive Control	Daudi, Raji, IM9, U266, YT, HUT-78, HUT-102, Jurkat, HL-60, THP-1, KG1a, human leukocytes. Human tonsil and lymph node.
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P19397
Uniprot	443057
Gene Id	963

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA; 0.05% azide. Also available WITHOUT BSA; azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF

Description

Functional Studies (Order Ab without Azide); Optimal dilution for a specific application should be determined., FACS ; IF

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	0.5-1μg/ml

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SKU	Supplier No.	Size	Your price	Quantity
BHA10206721	963-MSM1-P1ABX	100µg	$393.20		Add to Cart

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