Antigenic Surface Determinant Protein OA3 ; IAP ; Integrin Associated Protein ; Integrin Associated Signal Transducer ; MER6 ; OA3 ; Protein MER6 ; Rh Related Antigen
Categories
Primary Antibodies
Cellular Localization
Cell Surface, Cytoplasmic
Chromosome Location
3q13.12
Clonality
Monoclonal
Description
This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus.Ā CD47 may play a role in membrane transport and/or integrin dependent signal transduction.Ā
Host
Mouse
Immunogen
Recombinant human IAP protein (IAP/964); Intact CD47 purified from placenta (B6H12.2)
Positive Control
HUVEC, OVCA3 cells, Placenta, Brain and Ovarian Tumors
Reactivity
Human, Mouse
Recombinant
FALSE
Regulatory
RUO
Swissprot
Q08722
Uniprot
446414
Gene Id
961
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA; 0.05% azide. Also available WITHOUT BSA; azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
Functional Studies (Order Ab without Azide); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
1-2μg/ml
IHC-F
0.5-1μg/ml for 30 minutes at RT
Reviews of Anti-CD47 / IAP (Integrin Associated Protein) Antibody BHA10206706