20kDa homologous restriction factor (HRF20) ; Complement regulatory protein ; Human leukocyte antigen MIC11 ; MAC-inhibitory protein (MACIP) ; Membrane attack complex inhibition factor (MACIF) ; Membrane inhibitor of reactive lysis ; MIRL ; MSK21 ; Protectin ; T cell activating protein
Categories
Primary Antibodies
Cellular Localization
Cell Surface
Chromosome Location
11p13
Clonality
Monoclonal
Description
Reacts with human CD59, a 20kDa glycosyl phosphatidyl-inositol (GPI)-anchored cell surface protein. CD59 regulates complement-mediated cell lysis, and it is involved in lymphocyte signal transduction. This protein is a potent inhibitor of the complement membrane attack complex, whereby it binds complement C8 and/or C9 during the assembly of this complex, thereby inhibiting the incorporation of multiple copies of C9 into the complex, which is necessary for osmolytic pore formation. It inhibits formation of MAC, thus protecting cells from complement-mediated lysis. Genetic defects in GPI-anchor attachment, that cause a reduction or loss of CD59 and CD55 on erythrocytes produce the symptoms of the disease paroxysmal hemoglobinuria (PNH). This MAb is useful for study on GPI-anchored proteins, PNH and CD59 functions. CD59 is widely distributed on cells in all tissues. The expression of CD59 on erythrocytes is important for their survival.
Host
Mouse
Immunogen
Recombinant full-length human CD59 protein
Positive Control
Daudi, CEM, K562, HPB.ALL, Jurkat, Raji, and human lymphocytes. Tonsil, Cervix or Bladder.
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
P13987
Uniprot
278573; 709466; 710641
Gene Id
966
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
Functional Studies (Order Ab without Azide); (Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
0.5-1μg/ml
IHC-F
1-2μg/ml for 30 minutes at RT
No Description Available.
No Description Available.
Reviews of Anti-CD59 / Complement Regulatory Protein / Protectin Antibody BHA10206742