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Home / Primary Antibodies / Flow Cytometry (FACS)

Anti-CD20 / MS4A1 Antibody BHA10206331

SKU : BHA10206331

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NeoBiotechnologies, Inc.

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Mfr.No.	931-MSM1-P1ABX
Alternative Names	APY ; ATOPY ; B-lymphocyte antigen CD20 ; B-lymphocyte cell-surface antigen B1 ; Bp35 ; Fc epsilon receptor I beta chain ; Fc Fragment of IgE high affinity I receptor for beta polypeptide ; FCER1B ; High affinity immunoglobulin epsilon receptor subunit beta ; IgE Fc receptor subunit beta
Categories	Primary Antibodies
Cellular Localization	Cell Surface
Chromosome Location	11q12.2
Clonality	Monoclonal
Description	Recognizes a protein of 33-37kDa, identified as CD20 (Workshop V; Code CD20.12). B9E9 recognizes extracellular domain of CD20. The epitope is similar to or identical to that recognized by other CD20 antibodies including Leu-16 and B1. This MAb can be used for immunophenotyping of leukemia and malignant cells, B lymphocyte detection in peripheral blood, B cell localization in tissues and B lymphocyte purification by immunosorbent methods. CD20 is a non-Ig differentiation antigen of B-cells and its expression is restricted to normal and neoplastic B-cells, being absent from all other leukocytes and tissues. CD20 is expressed by pre B-cells and persists during all stages of B-cell maturation but is lost upon terminal differentiation into plasma cells. Protein passes through the membrane 4 times with both ends in cytoplasm and exposes one short and one longer loop to the external environment. CD20 is not glycosylated in resting B cells and its cytoplasmic domains are differentially phosphorylated upon activation. It acts as a calcium channel involved in B-cell activation and cell cycle progression.
Host	Mouse
Immunogen	Lymphoblastoid cell line Daudi
Positive Control	Daudi, Raji, and U266, and human lymphocytes. In normal lymph nodes and tonsils, CD20 is predominantly detected on B cells in germinal centers.
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P11836
Uniprot	712553
Gene Id	931

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA; 0.05% azide. Also available WITHOUT BSA; azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF

Description

Functional Studies (Order Ab without BSA & Azide); Optimal dilution for a specific application should be determined., FACS ; IF

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	0.5-1μg/ml

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SKU	Supplier No.	Size	Your price	Quantity
BHA10206331	931-MSM1-P1ABX	100µg	$393.20		Add to Cart

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