Anti-Caldesmon, HMW (h-Caldesmon) Antibody BHA10205929

Mfr.No.	800-RBM4-P1ABX
Alternative Names	CAD ; CALD1 ; Caldesmon 1 Isoform 1 ; Caldesmon 1 Isoform 2 ; Caldesmon 1 Isoform 3 ; Caldesmon 1 Isoform 4 ; Caldesmon 1 Isoform 5 ; CDM ; HCAD ; LCAD
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	7q33
Clonality	Monoclonal
Description	Recognizes a protein of 150kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120-150kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70-80kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.
Host	Rabbit
Immunogen	Recombinant human full-length CALD1 protein
Positive Control	Uterus, Blood vessels in all tissues, smooth muscle or leiomyosarcoma
Reactivity	Human, Rat
Recombinant	TRUE
Regulatory	RUO

Swissprot	Q05682
Uniprot	490203
Gene Id	800

Buffer	200μg/ml of Ab purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 1mM EDTA, pH 7.5-8.5, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F

Dilution

FACS	0.5-1μg/million cells
IF	1-2μg/ml
IHC-F	1-2μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10205929	800-RBM4-P1ABX	100µg	$393.20		Add to Cart

Anti-Caldesmon, HMW (h-Caldesmon) Antibody BHA10205929

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