Coagulation Factor VIII ; Factor VIII Related Antigen ; F8VWF ; von Willebrand Antigen 2 ; von Willebrand Disease (vWD)
Categories
Primary Antibodies
Cellular Localization
Cytoplasmic
Chromosome Location
12p13.31
Clonality
Monoclonal
Description
von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma and platelets. It acts as a carrier protein for Factor VIII and promotes platelet adhesion and aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity and availability for Factor VIII, including cleavage of the propeptide and formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposi s sarcoma and cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.
Host
Mouse
Immunogen
Recombinant fragment of human vWF protein (aa1815-1939) (exact sequence is proprietary)
Positive Control
HUVEC cells or Tonsil
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
P04275
Uniprot
440848
Gene Id
7450
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
ELISA, FACS, IF, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., ELISA ; FACS ; IF ; IHC-F
Dilution
ELISA
Use Ab at 2-4μg/ml for coating
FACS
0.5-1μg/million cells
IF
0.5-1μg/ml
IHC-F
1-2μg/ml for 30 minutes at RT
Reviews of Anti-von Willebrand Factor / Factor VIII Related-Ag Antibody BHA10205802