Anti-Villin Antibody BHA10205732

Mfr.No.	7429-MSM2-P1ABX
Alternative Names	VIL1 ; Villin-1 ; Villin1
Categories	Primary Antibodies
Cellular Localization	Cell Surface, Cytoplasmic
Chromosome Location	2q35
Clonality	Monoclonal
Description	Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
Host	Mouse
Immunogen	Recombinant human Villin fragment of 133 amino acid residues (aa179-311) (exact sequence is proprietary)
Positive Control	A549, HepG2 and HCT116 cells. Colon or Rectum.
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P09327
Uniprot	654595
Gene Id	7429

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA at 1.0mg/ml

Applications

FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution