This MAb recognizes human 17-26kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). TNF-alpha can be expressed as a 17kDa free molecule, or as a 26kDa membrane protein. TNF-alpha is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production. TNF alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.
Host
Rabbit
Immunogen
Recombinant full-length human TNF-alpha protein
Isotype
IgG Kappa
Positive Control
HeLa, HL-60, or A431 cells. Pancreas or Histiocytoma
Reactivity
Human, Rat
Recombinant
TRUE
Regulatory
RUO
Swissprot
P01375
Uniprot
241570
Gene Id
7124
Buffer
200μg/ml of Ab purified. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-FP
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-FP
Dilution
FACS
0.5-1μg/million cells
IF
1-2μg/ml
IHC-FP
4-8μg/ml for 30 min at RT
No Description Available.
No Description Available.
Reviews of Anti-TNF-alpha (Tumor Necrosis Factor alpha) Antibody BHA10205423