Anti-p53 Tumor Suppressor Protein Antibody BHA10205439

Mfr.No.	7157-MSM1-P1ABX
Alternative Names	Antigen NY-CO-13 ; BCC7 ; Cellular Tumor Antigen p53 ; LFS1 ; TP53 ; Transformation Related Protein 53 (TRP53) ; Tumor Protein p53 ; Tumor Suppressor p53
Categories	Primary Antibodies
Cellular Localization	Nuclear
Chromosome Location	17p13.1
Clonality	Monoclonal
Description	This MAb reacts with an N-terminal epitope (aa 16-25) of both wild type and mutated p53. Mutation and/or allelic loss of p53 is one of the causes of a variety of mesenchymal and epithelial tumors. If it occurs in the germ line, such tumors run in families. In most transformed and tumor cells the concentration of p53 is increased 51000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that of the wild-type (20 min). p53 Localizes in the nucleus, but is detectable at the plasma membrane during mitosis and when certain mutations modulate cytoplasmic/nuclear distribution. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas. High concentrations of p53 protein are transiently expressed in human epidermis and superficial dermal fibroblasts following mild ultraviolet irradiation. Positive nuclear staining with p53 antibody has been reported to be a negative prognostic factor in breast carcinoma, lung carcinoma, colorectal, and urothelial carcinoma. Anti-p53 positivity has also been used to differentiate uterine serous carcinoma from endometrioid carcinoma as well as to detect intratubular germ cell neoplasia.
Host	Mouse
Immunogen	Recombinant human wild-type p53 protein
Isotype	IgG2a
Positive Control	MDA-MB-231 Cells. Breast or Colon carcinoma
Reactivity	Human, Monkey, Dog, Hamster, Chicken
Recombinant	FALSE
Regulatory	RUO

Swissprot	P04637
Uniprot	654481
Gene Id	7157

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	1-2μg/ml
WB	0.5-1μg/ml
IHC-F	0.5-1μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10205439	7157-MSM1-P1ABX	100µg	$393.20		Add to Cart

Anti-p53 Tumor Suppressor Protein Antibody BHA10205439

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