BCH ; Benign chorea ; BHC ; Homeobox protein Nkx2.1 ; NK2 homeobox 1 ; NKX2.1 ; NKX2A ; TEBP ; Thyroid nuclear factor-1 ; Thyroid specific enhancer binding protein
Categories
Primary Antibodies
Cellular Localization
Nuclear
Chromosome Location
14q13.3
Clonality
Monoclonal
Description
Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland and the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate, and adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors, and malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates.ĀLoss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.
Host
Mouse
Immunogen
Rat full length TTF-1 recombinant protein
Isotype
IgG1 Kappa
Positive Control
MAD109, MLE-15, H441-4, or H345 cells. Normal thyroid or lung.
Reactivity
Human, Mouse, Rat
Recombinant
FALSE
Regulatory
RUO
Swissprot
P43699
Uniprot
94367
Gene Id
7080
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
0.5-1μg/ml
IHC-F
0.5-1μg/ml for 30 minutes at RT
Reviews of Anti-TTF-1 / NKX2.1 Antibody BHA10205339