This MAb recognizes extracellular epitope 2 within the N-terminal Ig domain of human CD147. It is expressed more intensely on thymocytes than on mature peripheral blood T cells. CD147 is important in spermatogenesis, embryo implantation, neural network formation, and tumor progression. It stimulates the production of interstitial collagenase, gelatinase A, stromelysin-1 and various metalloproteinases (MMPs) by fibroblasts. These enzymes are important factors in cancer invasion and metastasis.
Host
Mouse
Immunogen
Human T cell line Molt 13
Isotype
IgG1
Positive Control
HSB2 cells. Renal Cell, Ovarian carcinoma or Melanoma
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
P35613
Uniprot
501293
Gene Id
682
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
Inhibits Homotypic Aggregation, Adhesion to Matrix Proteins, and Migation through Matrigel (Order Ab without BSA/Azide); (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
1:100-1:200
IHC-F
0.5-1μg/ml for 30 minutes at RT
Reviews of Anti-CD147 / EMMPRIN / Neurothelin Antibody BHA10205108