Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock and tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors and cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts and other cell lines, activating polymorphonuclear neutrophils and osteoclasts, and induction of interleukin 1, prostaglandin E2 and collagenase production.
Host
Mouse
Immunogen
A hexadecapeptide corresponding to aa115-130 (NGVELRDNQLVVPSEG) of human TNF-, conjugated to thyroglobulin
Isotype
IgM Kappa
Positive Control
HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil
Reactivity
Human, Mouse, Rat, Rabbit, Cat, Dog, Zebrafish
Recombinant
FALSE
Regulatory
RUO
Swissprot
P01375
Uniprot
241570
Gene Id
7124
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-FP
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris Buffer with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-FP
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
1-2μg/ml
IHC-FP
2-4μg/ml for 30 min at RT
Reviews of Anti-TNF-alpha (Tumor Necrosis Factor alpha) Antibody BHA10205382