This antibody reacts with the TGF alpha and shows no cross-reaction with EGF and the neuropeptide synenkephalin. The staining with this MAb is completely blocked by the peptide used for raising this antibody. TGFĪ � (aa50) is a growth factor with 33% homology to EGF, binds to EGFR, activates tyrosine phosphorylation of the receptor, and stimulates cell proliferation. It plays a role in tumor initiation by inducing the reversible transformed phenotype.
Host
Mouse
Immunogen
A 10-amino acid synthetic peptide (aa34-43; PPVAAAVVSH) from human TGFα.
Isotype
IgG1 Kappa
Positive Control
Jurkat or Ramos cells. Testicular Carcinoma
Reactivity
Human, Rabbit, Zebrafish
Recombinant
FALSE
Regulatory
RUO
Swissprot
P01135
Uniprot
170009
Gene Id
7039
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-FP
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-FP
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
1-2μg/ml
IHC-FP
2-4μg/ml for 30 min at RT
Reviews of Anti-TGF-alpha (Transforming Growth Factor alpha) Antibody BHA10205259