Anti-Cyclin D1 Antibody BHA10204684

Mfr.No.	595-MSM2-P1ABX
Alternative Names	B cell CLL/lymphoma 1 ; B cell leukemia 1 ; B-cell lymphoma 1 protein ; BCL-1 oncogene ; CCND1 protein ; CCND1/FSTL3 fusion gene ; CCND1/IGHG1 fusion gene CCND1/IGLC1 fusion gene ; CCND1/PTH fusion gene ; cD1 ; Cyl 1
Categories	Primary Antibodies
Cellular Localization	Nuclear
Chromosome Location	11q13.2
Clonality	Monoclonal
Description	Recognizes a protein of 36kDa, identified as cyclin D1. Cyclin D1, one of the key cell cycle regulators, is a putative proto-oncogene overexpressed in a wide variety of human neoplasms. This antibody neutralizes the activity of cyclin D1 in vivo. About 60% of mantle cell lymphomas (MCL) contain a t(11; 14)(q13; q32) translocation resulting in over-expression of cyclin D1. This antibody is useful in identifying mantle cell lymphomas (cyclin D1 positive) from CLL/SLL and follicular lymphomas (cyclin D1 negative). Occasionally, hairy cell leukemia and plasma cell myeloma weakly express Cyclin D1.
Host	Mouse
Immunogen	Recombinant human CCND1 protein
Isotype	IgG2a Kappa
Positive Control	T47D, ZR75, BT474, SKBR3, MCF-7, MDA-MB-453, HT29, Ramos, Jurkat or A431 cells. Mantle cell lymphoma, breast or bladder carcinomas.
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P24385
Uniprot	523852; 667996
Gene Id	595

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution