S100-A9 ; MRP14 ; calgranulin-B ; calprotectin L1H subunit ; leukocyte L1 complex heavy chain ; migration inhibitory factor-related protein 14 ; S100 calcium-binding protein A9 (calgranulin B)
Categories
Primary Antibodies
Cellular Localization
Cytoplasmic
Chromosome Location
1q21
Clonality
Monoclonal
Description
Recognizes a protein of14kDa, identified as MRP-14 (also known as Calgranulin B or S100AA9). It comprises 60% of the cytoplasmic protein fraction of circulating polymorphonuclear granulocytes and is also found in monocytes, macrophages and ileal tissue eosinophils. Peripheral blood monocytes carry the antigen extra- and intracellularly, neutrophils only intracellularly. It is a potent chemotactic factor for neutrophils. Plasma concentrations are elevated in diseases associated with increased neutrophil activity, like inflammatory bowel disease. Granulocytes terminate their existence after transmigration through the intestinal wall. Therefore, it is also detectable in feces. Elevated levels have been observed in body fluids such as plasma, saliva, gingival crevicular fluid, stools, and synovial fluid during infection and inflammatory conditions. This MAb reacts with neutrophils, monocytes, and macrophages, and has been shown as an important marker for identifying macrophages in tissue sections.
Host
Mouse
Immunogen
Recombinant human MRP14 protein
Isotype
IgM Kappa
Positive Control
Tonsil, lymph node, or spleen
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
P06702
Uniprot
112405
Gene Id
6280
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate. Prepared in 1mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
0.5-1μg/ml
IHC-F
0.5-1μg/ml for 30 minutes at RT
Reviews of Anti-Myeloid-Related Proteins 14 (MRP14) Antibody BHA10204824