Apoptosis regulator Bcl-X ; B cell lymphoma 2 like ; BCL XL/S ; Bcl-2-like protein 1 ; Bcl2-L-1 ; BCL2L ; Bcl2l1 ; BCLX ; BclXL ; BclXs
Categories
Primary Antibodies
Cellular Localization
Cytoplasmic, Nuclear membrane
Chromosome Location
20q11.21
Clonality
Monoclonal
Description
Recognizes a protein of 27kDa, identified as the Bcl-X protein. This MAb shows no cross-reaction with Bcl-2 or Bax protein. Bcl-X has two isoforms, Bcl-XL (long), a 241 amino acid protein which suppresses cell death. And Bcl-XS (short), a 178 amino acid protein lacking a 63 amino acid domain which functions as a dominant inhibitor of Bcl-2. This MAb reacts with both Bcl-XS and Bcl-XL proteins.
Host
Mouse
Immunogen
A synthetic peptide, aa 3-14 (Cys-QSNRELVVDFLS) of human Bcl-X protein (BX006 & 2H12)
Isotype
IgG
Positive Control
Jurkat, K562, HL-60, or HeLa Cells. Reed-Sternberg cells in Hodgkin s lymphoma.
Reactivity
Human, Mouse, Rat, Pig
Recombinant
FALSE
Regulatory
RUO
Swissprot
Q07817
Uniprot
516966
Gene Id
598
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, WB, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F