B220 ; CD45R ; GP180 ; Leukocyte common antigen (LCA) ; Loc ; Ly-5 ; Lyt-4 ; Protein tyrosine phosphatase receptor type C (PTPRC) ; Receptor-type tyrosine-protein phosphatase C ; T200 glycoprotein
Categories
Primary Antibodies
Cellular Localization
Cell Surface
Chromosome Location
1q31.3
Clonality
Monoclonal
Description
Recognizes a protein of 205kDa-220kDa, identified as CD45RA (Workshop III). CD45RA is isoforms of the human leukocyte common antigen (CD45). Human CD45 contains three exons which encode peptide segments designated A, B and C, respectively. The differential splicing of the exons generates at least five isoforms, ABC, AB, BC, B and O. This antibody reacts with ABC and BC isoforms. CD45RA is expressed on 40-50% of peripheral CD4+ T-cells, 50% of peripheral CD8+ T-cells, B-cells, and leukemic B-cell lines. T-cells expressing CD45RA are naive or virgin T-cells. T-cells expressing CD45RO are memory T-cells. CD45RA and CD45RO define complementary, predominantly non-overlapping populations of resting peripheral T-cells. This MAb is useful in study on the subpopulation of CD4+ or CD8+ T-cells. It can especially be used to differentiate T-cell lymphomas (CD45RO +ve) from B cell lymphomas (CD45RA +ve).
Host
Mouse
Immunogen
Stimulated human leukocytes
Isotype
IgG1 Kappa
Positive Control
Daudi, Raji, IM9 and human lymphocytes. Human lymph nodes and tonsils.
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
P08575
Uniprot
654514
Gene Id
5788
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F