Anti-Actin, Smooth Muscle Antibody BHA10204630

Mfr.No.	59-MSM3-P1ABX
Alternative Names	ACTA2 ; Actin Alpha 2 Smooth Muscle Aorta ; Actin Aortic Smooth Muscle ; Actin Vascular Smooth Muscle ; ACTSA ; ACTVS ; Alpha 2 Actin ; Alpha Actin 2 ; Alpha Cardiac Actin ; Alpha Smooth Muscle
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	10q23.31
Clonality	Monoclonal
Description	Actin is a major component of the cytoskeleton and is present in most cell types. It is highly specific to actin from smooth muscles. This MAb does not stain cardiac or skeletal muscle; however, it does stain myofibroblasts and myoepithelial cells. This antibody could be used together with anti-muscle specific actin and myogenin in making a diagnosis of smooth muscle and skeletal muscle tumors. In most cases of rhabdomyosarcoma, this antibody yields negative results whereas anti-muscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with anti-muscle specific actin and anti-smooth muscle actin and are negative with anti-myogenin.
Host	Mouse
Immunogen	N-Terminal decapeptide of alpha smooth muscle isoform of actin and conjugated to KLH (1A4); Recombinant full-length human ACTA2 protein (ACTA2/791)
Isotype	IgG
Positive Control	Blood vessels in all tissues, smooth muscle or leiomyosarcoma
Reactivity	Human, Rat
Recombinant	FALSE
Regulatory	RUO

Swissprot	P62736
Uniprot	500483
Gene Id	59

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F

Dilution