It recognizes a protein of 54kDa, which is identified antitrypsin (AAT). The immunohistochemical staining of AAT is useful in identification of benign and malignant hepatic tumors and yolk sac carcinomas. Positive staining for AAT is also used in detection of benign and malignant lesions of histiocytic nature. This antibody is may also useful tool in the screening of patients with cryptogenic cirrhosis or other forms of liver disease with fibrosis of uncertain origin.
Host
Mouse
Immunogen
Recombinant human Alpha-1-Antitrypsin (AAT) protein fragment (exact sequence is proprietary)
Isotype
IgG1
Positive Control
HepG2 Cells. Tonsil, Hepatocellular Carcinoma or Histiocytoma.
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
P01009
Uniprot
525557
Gene Id
5265
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, WB, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; WB ; IHC-F
Dilution
FACS
0.5-1μg/million cells
IF
0.5-1μg/ml
WB
0.5-1.0μg/ml
WB
0.5-1.0μg/ml
IHC-F
0.5-1μg/ml for 30 minutes at RT
No Description Available.
No Description Available.
Reviews of Anti-Alpha-1-Antitrypsin (SERPINA1) Antibody BHA10204100