T200 ; GP180 ; LCA ; Leukocyte Common Antigen ; LY5 ; Receptor-type tyrosine-protein phosphatase C ; T200 glycoprotein
Categories
Primary Antibodies
Cellular Localization
Cell Surface
Chromosome Location
1q31.3
Clonality
Monoclonal
Description
Recognizes a 180-185kDa protein, identified as isoform of leukocyte common antigen (CD45RO) (4th Leucocyte Typing Workshop: Code No. N31). The epitope recognized by this antibody is sensitive to neuraminidase digestion. This antibody reacts with mature activated T-cells, most thymocytes, and a sub-population of resting T-cells within both CD4 and CD8 subsets. It shows no reactivity with normal B or natural killer cells, but reacts with granulocytes and monocytes. Reportedly, it is useful to identify T-cell lymphomas and leukemias. It rarely stains NK cells or B-cell lymphomas.
Host
Mouse
Immunogen
Cultured human T-cells from an IL-2-dependent T-cell line (CA1)
Isotype
IgG2a Kappa
Positive Control
CCRF-CEM, Jurkat or MOLT-4 cells. Paracortex in a tonsil or a reactive lymph node.
Reactivity
Human, Chimpanzee, Common Marmoset
Recombinant
FALSE
Regulatory
RUO
Swissprot
P08575
Uniprot
654514
Gene Id
5788
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F