Anti-MAML3 (Mastermind Like Transcriptional Coactivator 3) Antibody BHA10204315

Mfr.No.	55534-MSM1-P1ABX
Alternative Names	CAGH3 ; ERDA3 ; GDN ; MAML3 ; Mastermind-like protein 3 ; TNRC3
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	4q28
Clonality	Monoclonal
Description	MAML3 (mastermind-like protein 3) is a nuclear speckle protein that acts as a transcriptional coactivator for Notch receptors. The Notch signaling pathway influences cell fate by regulating the ability of precursor cells to properly respond to developmental signals. MAML3 is a member of the mastermind-like family of proteins that are human homologs of the Drosophila melanogaster mastermind protein. Through its N-terminal region, MAML3 interacts with the ankyrin repeats of the Notch proteins Notch 1, Notch 2, Notch 3 and Notch 4.This interaction leads to formation of a DNA-binding complex with the Notch proteins and RBP-Jκ; a complex that can then induce HES1 gene expression. While the N-terminal domain of MAML3 is essential for proper Notch binding, the C-terminal domain of MAML3 is essential for transcriptional activation. Due to its involvement in cell signaling and transcriptional activation, upregulation of MAML3 is thought to be involved in oncogenesis.
Host	Mouse
Immunogen	Recombinant human full-length MAML3 protein
Isotype	IgG1
Positive Control	MCF-7 cells. Pancreas or Placenta. Cervix or Colon Carcinoma.
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	Q96JK9 (Human)
Uniprot	586165 (Human)
Gene Id	55534 (Human)

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, IHC-F

Description

(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0 for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F

Dilution