Adrenocorticotropin ; alpha or beta or gamma Melanocyte Stimulating Hormone (MSH) or Melanotropin ; beta-Endorphin ; beta or gamma Lipotropin (LPH) ; CLIP ; Met Enkephalin ; POC ; POMC
Categories
Primary Antibodies
Cellular Localization
Cytoplasmic (Secreted)
Chromosome Location
2p23.3
Clonality
Monoclonal
Description
ACTH (same as Corticotropin) is a 39 amino acid active peptide produced by the anterior pituitary. This MAb is specific to Synacthen (aa1-24 of ACTH); does not react with CLIP (aa17-39 of ACTH). POMC (pro-opiomelanocortin or corticotropin-lipotropin) is a 267 amino acid polypeptide hormone precursor that goes through extensive, tissue-specific posttranslational processing by convertases. POMC is cleaved into ten hormone chains named NPP, ACTH, alpha-MSH (Melanocyte Stimulating Hormone), beta-MSH, gamma-MSH, CLIP (corticotropin-like intermediary peptide), Lipotropin-beta, Lipotropin-gamma, beta-endorphin and Met-enkephalin. ACTH is also produced by cells of immune system (T-cells, B-cells, and macrophages) in response to stimuli associated with stress. Anti-ACTH is a useful marker in classification of pituitary tumors and the study of pituitary disease. It reacts with ACTH-producing cells (corticotrophs). It also may react with other tumors (e.g. some small cell carcinomas of the lung) causing paraneoplastic syndromes by secreting ACTH.
Host
Mouse
Immunogen
N-terminal fragment of human ACTH conjugated to KLH
Isotype
IgG1 Kappa
Positive Control
Normal pituitary gland or pituitary tumor.
Reactivity
Human, Mouse, Rat
Recombinant
TRUE
Regulatory
RUO
Swissprot
P01189
Uniprot
1897
Gene Id
5443
Buffer
200μg/ml of recombinant MAb purified by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
ELISA, FACS, IF, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., ELISA ; FACS ; IF ; IHC-F
Dilution
ELISA
For coating, order Ab without BSA
FACS
0.5-1μg/million cells
IF
0.5-1μg/ml
IHC-F
0.5-1.0μg/ml for 30 minutes at RT
Reviews of Anti-ACTH (Adrenocorticotrophic Hormone) (N-Terminal) Antibody BHA10204208