Anti-TDP2 / EAPII Antibody BHA10203992

Mfr.No.	51567-MSM1-P1ABX
Alternative Names	5'-Tyr-DNA phosphodiesterase ; EAP2 ; EAPII ; ETS1-associated protein 2 ; ETS1-associated protein II ; TRAF and TNF receptor-associated protein ; TTRAP ; Tyr-DNA phosphodiesterase 2 ; Tyrosyl-DNA phosphodiesterase 2 (TDP2)
Antigen	TDP2 / EAPII
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic, Nuclear
Clonality	Monoclonal
Conjugate	Unconjugated
Description	This MAb recognizes a protein of 41kDa, which is identified as TDP2. It is a member of a superfamily of divalent cation-dependent phosphodiesterases. The encoded protein associates with CD40, tumor necrosis factor (TNF) receptor-75 and TNF receptor associated factors (TRAFs), and inhibits nuclear factor-kappa-B activation. This protein has sequence and structural similarities with APE1 endonuclease, which is involved in both DNA repair and the activation of transcription factors. DNA repair enzyme that can remove a variety of covalent adducts from DNA through hydrolysis of a 5'-phosphodiester bond, giving rise to DNA with a free 5' phosphate. Catalyzes the hydrolysis of dead-end complexes between DNA and the topoisomerase 2 (TOP2) active site tyrosine residue. Hydrolyzes 5'-phosphoglycolates on protruding 5' ends on DNA double-strand breaks (DSBs) due to DNA damage by radiation and free radicals. The 5'-tyrosyl DNA phosphodiesterase activity can enable the repair of TOP2-induced DSBs without the need for nuclease activity, creating a 'clean' DSB with 5'-phosphate termini that are ready for ligation. Has also 3'-tyrosyl DNA phosphodiesterase activity, but less efficiently and much slower than TDP1. May also act as a negative regulator of ETS1 and may inhibit nuclear factor-kappa-B activation.
Host	Mouse
Immunogen	Recombinant human full-length TDP2 protein
Isotype	IgG2b Kappa
Positive Control	Jurkat cells. Lung, Duodenum or Small Intestinal Carcinoma.
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	O95551 (Human)
Uniprot	728795 (Human)Human Gene SymbolTDP2Hu Chromosome Location6p22.3-22.1
Gene Id	51567 (Human)

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F

Dilution