Cyclin ; DNA polymerase delta auxiliary protein ; Mutagen-sensitive 209 protein ; PCNAR ; Polymerase delta accessory protein
Categories
Primary Antibodies
Cellular Localization
Cytoplasmic, Nuclear
Chromosome Location
20p12.3
Clonality
Monoclonal
Description
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase and declining during G2 and M phases. This MAb is excellent for multiple applications.
Host
Mouse
Immunogen
Rat PCNA/Protein A fusion protein
Isotype
IgG2a Kappa
Positive Control
Tonsil or reactive lymph node
Reactivity
Human, Monkey, Pig, Mouse, Rat, Chicken, Zebrafish, Drosophila melanogaster, Yeast
Recombinant
FALSE
Regulatory
RUO
Swissprot
P12004
Uniprot
147433; 728886
Gene Id
5111
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, WB, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
0.5-1μg/ml
WB
0.5-1μg/ml
IHC-F
0.25-0.5μg/ml for 30 minutes at RT
Reviews of Anti-PCNA (Proliferating Cell Nuclear Antigen) Antibody BHA10203960