Anti-MyoD1 Antibody BHA10203664

Mfr.No.	4654-MSM3-P1BX
Alternative Names	bHLHc1 ; Class C basic helix-loop-helix protein 1 ; Myoblast determination protein 1 ; Myogenic differentiation 1 ; Myogenic factor 3 (Myf-3) ; Myogenin D1 ; PUM
Categories	Primary Antibodies
Cellular Localization	Nuclear
Chromosome Location	11p15.1
Clonality	Monoclonal
Description	Recognizes a phosphor-protein of 45kDa, identified as MyoD1. This MAb does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilm s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewing s sarcomas and alveolar soft part sarcomas.
Host	Mouse
Immunogen	Recombinant mouse MyoD1 protein (5.8A); Recombinant human MyoD1 protein (MYD712)
Isotype	IgG
Positive Control	Rhabdomyosarcoma
Reactivity	Human, Mouse, Rat, Chicken
Recombinant	FALSE
Regulatory	RUO

Swissprot	P15172
Uniprot	181768
Gene Id	4654

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA at 1.0mg/ml

Applications

FACS, IF, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	0.5-1μg/ml
IHC-F	0.5-1μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10203664	4654-MSM3-P1BX	100µg	$393.20		Add to Cart

Anti-MyoD1 Antibody BHA10203664

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