68kDa neurofilament protein ; Light molecular weight neurofilament protein ; NEFL ; Neurofilament light polypeptide 68kDa ; Neurofilament light polypeptide ; Neurofilament protein ; light chain ; Neurofilament subunit NF-L ; Neurofilament triplet L protein ; NF-L
Categories
Primary Antibodies
Cellular Localization
Cytoplasmic
Chromosome Location
8p21.2
Clonality
Monoclonal
Description
This MAb reacts with a 68kDa protein, identified as light sub-unit of neurofilaments (NF-L). Neurofilaments make up the main structural elements of axons and dendrites and are found in neurons, peripheral nerves, and sympathetic ganglion cells. Neurofilaments consist of three major subunits with molecular weights of 68kDa (NF-L), 160kDa (NF-M) and 200kDa (NF-H). Anti-neurofilament stains a number of neural, neuroendocrine, and endocrine tumors. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas, and neuroblastomas stain positively for anti-neurofilament. Neurofilaments are also present in paragangliomas as well as adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilament.
Host
Mouse
Immunogen
Neurofilament purified from human brain
Isotype
IgG1
Positive Control
Brain, Neuroblastoma
Reactivity
Human, Rat, Pig, Cow, Chicken
Recombinant
FALSE
Regulatory
RUO
Swissprot
P07196
Uniprot
521461
Gene Id
4747
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
IF, FACS, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., IF ; FACS ; IHC-F
Dilution
IF
1-2μg/ml
FACS
0.5-1μg/million cells in 0.1ml
IHC-F
0.25-0.5μg/ml for 30 minutes at RT
Reviews of Anti-Neurofilament (NF-L) Antibody BHA10203802