Anti-Nucleolin Antibody BHA10203748

Mfr.No.	4691-MSM1X-P1ABX
Alternative Names	NCL ; Nucl ; Nucleolin ; Protein C23
Antigen	Nucleolin
Categories	Primary Antibodies
Cellular Localization	Nucleoli
Chromosome Location	2q37.1
Clonality	Monoclonal
Conjugate	Unconjugated
Description	Recognizes a protein of ~76kDa, which is identified as Nucleolin (NCL). It is the major nucleolar phosphoprotein of growing eukaryotic cells. NCL is located mainly in dense fibrillar regions of the nucleolus. It is found associated with intranucleolar chromatin and pre-ribosomal particles. Human NCL gene consists of 14 exons with 13 introns and spans approximately 11kb. It induces chromatin decondensation by binding to histone H1. It is thought to play a role in pre-rRNA transcription and ribosome assembly.ĀThis MAb can be used to stain the nucleoli in cell or tissue preparations and can be used as a marker of the nucleoli in subcellular fractions. It produces a speckled pattern in the nuclei of cells of normal and malignant cells and may be used to stain the nucleoli of cells in fixed or frozen tissue sections. It can be used with paraformaldehyde fixed frozen tissue or cell preparations and formalin fixed, paraffin-embedded tissue sections.
Host	Mouse
Immunogen	Recombinant human NCL protein
Isotype	IgG1 Kappa
Positive Control	All human Cells. Testis, Ovary, Uterus, Endometrial or Hodgkin s lymphoma
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P19338
Uniprot	79110
Gene Id	4691

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, WB, IHC-F

Description

(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	0.5-1μg/ml
WB	0.5-1μg/ml
IHC-F	0.5-1.0μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10203748	4691-MSM1X-P1ABX	100µg	$393.20		Add to Cart

Anti-Nucleolin Antibody BHA10203748

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