bHLHc3 ; cb553 ; Class C basic helix-loop-helix protein 3 ; Myf-4 ; MYF4 ; MYOG ; Myogenic factor 4
Categories
Primary Antibodies
Cellular Localization
Nuclear
Chromosome Location
1q32.1
Clonality
Monoclonal
Description
Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms� tumor.
Host
Mouse
Immunogen
Human myogenin recombinant protein (MGN185); Rat myogenin recombinant fragment containing amino acid 30-224 (F5D)
Isotype
IgG
Positive Control
Rh-30 cells. Skeletal muscle or rhabdomyosarcoma.
Reactivity
Human, Mouse, Rat, Cat, Pig
Recombinant
FALSE
Regulatory
RUO
Swissprot
P15173
Uniprot
2830
Gene Id
4656
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F