bHLHc1 ; Class C basic helix-loop-helix protein 1 ; Myoblast determination protein 1 ; Myogenic differentiation 1 ; Myogenic factor 3 (Myf-3) ; Myogenin D1 ; PUM
Categories
Primary Antibodies
Cellular Localization
Nuclear
Chromosome Location
11p15.1
Clonality
Monoclonal
Description
Recognizes a phosphor-protein of 45kDa, identified as MyoD1. The epitope of this MAb maps between amino acid 180-189 in the C-terminal of mouse MyoD1 protein. It does not cross react with myogenin, Myf5, or Myf6. Antibody to MyoD1 labels the nuclei of myoblasts in developing muscle tissues. MyoD1 is not detected in normal adult tissue, but is highly expressed in the tumor cell nuclei of rhabdomyosarcomas. Occasionally nuclear expression of MyoD1 is seen in ectomesenchymoma and a subset of Wilmā€™s tumors. Weak cytoplasmic staining is observed in several non-muscle tissues, including glandular epithelium and also in rhabdomyosarcomas, neuroblastomas, Ewingā€™s sarcomas and alveolar soft part sarcomas.
Host
Mouse
Immunogen
Recombinant mouse MyoD1 protein
Isotype
IgG1 Kappa
Positive Control
Rhabdomyosarcoma
Reactivity
Human, Mouse, Rat, Chicken
Recombinant
FALSE
Regulatory
RUO
Swissprot
P15172
Uniprot
181768
Gene Id
4654
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F