Anti-MUC1 / EMA / CD227 Antibody BHA10203440

Mfr.No.	4582-MSM5-P1ABX
Alternative Names	Breast carcinoma-associated antigen DF3 ; CA15-3 ; Carcinoma-associated mucin Episialin ; Epithelial Membrane Antigen ; H23AG ; KL-6 ; MAM6 ; MUC-1 ; MUC-1/SEC ; MUC-1/X
Categories	Primary Antibodies
Cellular Localization	Cell Surface, Cytoplasmic
Chromosome Location	1q22
Clonality	Monoclonal
Description	This MAb reacts with MUC1. The dominant epitope of this MAb has not yet been determined. MUC1 is a large cell surface mucin glycoprotein expressed by most glandular and ductal epithelial cells and some hematopoietic cell lineages. It is expressed on most secretory epithelium, including mammary gland and some hematopoietic cells. It is expressed abundantly in lactating mammary glands and over expressed abundantly in >90% breast carcinomas and metastases. Transgenic MUC1 has been shown to associate with all four c-erbB receptors and localize with c-erbB1 (EGFR) in lactating glands. The MUC1 gene contains seven exons and produces several different alternatively spliced variants. The major expressed form of MUC1 uses all seven exons and is a type 1 transmembrane protein with a large extracellular tandem repeat domain. The tandem repeat domain is highly O glycosylated and alterations in glycosylation have been shown in epithelial cancer cells. Antibody to EMA is useful as a pan-epithelial marker for detecting early metastatic loci of carcinoma in bone marrow or liver.
Host	Mouse
Immunogen	Human milk-fat globule membranes (HMFGM)
Isotype	IgG1 Kappa
Positive Control	MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.
Reactivity	Human, Mouse
Recombinant	FALSE
Regulatory	RUO

Swissprot	P15941
Uniprot	89603
Gene Id	4582

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

ELISA, FACS, IF, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., ELISA ; FACS ; IF ; IHC-F

Dilution