Anti-MUC1 / EMA / CD227 Antibody BHA10203375

Mfr.No.	4582-MSM11-P1ABX
Alternative Names	Breast carcinoma-associated antigen DF3 ; CA15-3 ; Carcinoma-associated mucin Episialin ; Epithelial Membrane Antigen ; H23AG ; KL-6 ; MAM6 ; MUC-1 ; MUC-1/SEC ; MUC-1/X
Categories	Primary Antibodies
Cellular Localization	Cell Surface, Cytoplasmic
Chromosome Location	1q22
Clonality	Monoclonal
Description	MAb VU-2G7 reacts with MUC1, a large transmembrane glycoprotein expressed on the ductal surface of normal glandular epithelia. The dominant epitope of VU-2G7 has not been established with �epitope fingerprinting�. The?extra cellular domain of MUC1 largely consists of a highly conserved, O-glycosylated 20 amino acids tandem repeat which can occur 30-100 times per molecule depending on the length of the allele involved. In the vast majority of human carcinomas this protein is up regulated and poorly glycosylated and appears on the cell surface in a non-polarized fashion.
Host	Mouse
Immunogen	Synthetic glycosylated MUC1 60mer tandem repeat NH2-(HGVTSAPDT(GalNAc)RPAPGSTAPPAHG)3- COOH, conjugated to bovine serum albumin
Isotype	IgG1 Kappa
Positive Control	MCF-7 or MDA-231 cells. Breast, colon, ovarian, endometrial carcinoma.
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P15941
Uniprot	89603
Gene Id	4582

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA; 0.05% azide. Also available WITHOUT BSA; azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, IHC-FP

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-FP

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	1-2μg/ml
IHC-FP	0.5-1μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10203375	4582-MSM11-P1ABX	100µg	$393.20		Add to Cart

Anti-MUC1 / EMA / CD227 Antibody BHA10203375

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