Anti-Microphthalmia Transcription Factor (MITF) Antibody BHA10203310

Mfr.No.	4286-MSM2-P1ABX
Alternative Names	BHLHE32 ; Class E basic helix-loop-helix protein 32 (bHLHe32) ; CMM8 ; Mi ; Microphthalmia-associated transcription factor ; MITF ; WS2 ; WS2A
Categories	Primary Antibodies
Cellular Localization	Nuclear
Chromosome Location	3p14.1
Clonality	Monoclonal
Description	MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. This MAb recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
Host	Mouse
Immunogen	Recombinant full-length human MiTF protein
Isotype	IgG1 Kappa
Positive Control	Jurkat, A-431, HeLa or 501 Mel human melanoma cells or Melanoma
Reactivity	Human, Dog
Recombinant	FALSE
Regulatory	RUO

Swissprot	O75030
Uniprot	166017; 618266
Gene Id	4286

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	0.5-1μg/ml
IHC-F	0.5-1.0μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10203310	4286-MSM2-P1ABX	100µg	$393.20		Add to Cart

Anti-Microphthalmia Transcription Factor (MITF) Antibody BHA10203310

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