Anti-CD99 / MIC2 Antibody BHA10203277

Mfr.No.	4267-MSM3-P1ABX
Alternative Names	E2 antigen ; MIC 2X ; MIC 2Y ; MIC2 ; Protein MIC2 ; Surface antigen MIC2 ; T-cell surface glycoprotein E2
Categories	Primary Antibodies
Cellular Localization	Cell Surface
Chromosome Location	Xp22.33
Clonality	Monoclonal
Description	Recognizes a sialoglycoprotein of 27-32kDa, identified as CD99, or MIC2 gene product, or E2 antigen. MIC2 gene is located in the pseudo-autosomal region of the human X and Y chromosome. MIC2 gene encodes two distinct proteins, which are produced by alternative splicing of the CD99 gene transcript and are identified as bands of 30 and 32kDa (p30/32).ĀAlthough its function is not fully understood, CD99 is implicated in various cellular processes including homotypic aggregation of T cells, upregulation of T cell receptor and MHS molecules, apoptosis of immature thymocytes and leukocyte diapedesis.ĀCD99 is expressed on the cell membrane of some lymphocytes, cortical thymocytes, and granulosa cells of the ovary. Most pancreatic islet cells, Sertoli cells of the testis, and some endothelial cells express this antigen. Mature granulocytes express very little or no CD99. MIC2 is strongly expressed on Ewing s sarcoma cells and primitive peripheral neuroectodermal tumors.
Host	Mouse
Immunogen	Recombinant full-length human MIC2 protein
Isotype	IgG1 Kappa
Positive Control	MOLT-4 cells. Pancreas or Ewing s sarcoma
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P14209
Uniprot	653349
Gene Id	4267

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F

Dilution