BHLHE32 ; Class E basic helix-loop-helix protein 32 (bHLHe32) ; CMM8 ; Mi ; Microphthalmia-associated transcription factor ; MITF ; WS2 ; WS2A
Categories
Primary Antibodies
Cellular Localization
Nuclear
Chromosome Location
3p14.1
Clonality
Monoclonal
Description
MITF (microphthalmia transcription factor) is a basic helix-loop-helix-leucine-zipper (bHLH-Zip) transcription factor that regulates the development and survival of melanocytes and retinal pigment epithelium, and also is involved in transcription of pigmentation enzyme genes such as tyrosinase TRP1 and TRP2. MITF has been shown to be phosphorylated by MAP kinase in response to c-kit activation, resulting in upregulation of MITF transcriptional activity. Mutations of the MITF gene are associated with the autosomal dominant hereditary deafness and pigmentation condition, Waardenburg Syndrome type 2A. Multiple isoforms of MITF exist, including MITF-A, MITF-B, MITF-C, MITF-H, and MITF-M, which differ in the amino-terminal domain and in their expression patterns. The MITF-M isoform is restricted to the melanocyte cell lineage. Anti-MITF, D5, recognizes a nuclear protein, which is expressed in the majority of primary and metastatic epithelioid malignant melanomas as well as in normal melanocytes, benign nevi and dysplastic nevi.
Host
Mouse
Immunogen
NH2 terminus fragment of human Mi protein
Isotype
IgG1 Kappa
Positive Control
Jurkat, A-431, HeLa or 501 Mel human melanoma cells or Melanoma
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
O75030
Uniprot
166017; 618266
Gene Id
4286
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
0.5-1μg/ml
IHC-F
0.5-1.0μg/ml for 30 minutes at RT
Reviews of Anti-Microphthalmia Transcription Factor (MITF) Antibody BHA10203304