Anti-Involucrin Antibody BHA10202534

Mfr.No.	3713-MSM1X-P1ABX
Alternative Names	IVL
Categories	Primary Antibodies
Cellular Localization	Cytoplasmic
Chromosome Location	1q21.3
Clonality	Monoclonal
Description	It recognizes a protein of 66kDa-170kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120kDa protein. It stains the involucrin in a variety of sizes: 170kDa in MCF-7 cells, a doublet of ~115kDa and 150kDa in gorilla and owl monkey, 66kDa in dog, and a doublet of 105kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal; expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.
Host	Mouse
Immunogen	Purified involucrin from human keratinocytes
Isotype	IgG1 Kappa
Positive Control	MCF-7 cells. Localized to upper spinous and granular layers in normal skin.
Reactivity	Human, Gorilla, Owl monkey, Pig, Dog
Recombinant	FALSE
Regulatory	RUO

Swissprot	P07476
Uniprot	516439
Gene Id	3713

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	1.0mg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Applications

FACS, IF, IHC-F

Description

(Staining of formalin-fixed tissues is enhanced by digestion with trypsin or Protease XXV at 1mg/ml PBS for 5 min at 37 �C); (Note than enzyme digestion is better than citrate buffer based epitope unmasking); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	1-2μg/ml
IHC-F	0.1-0.2μg/ml for 30 min at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10202534	3713-MSM1X-P1ABX	100µg	$393.20		Add to Cart

Anti-Involucrin Antibody BHA10202534

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