BCIE ; BIE ; EHK ; Keratin Type I Cytoskeletal 10 ; KRT10
Categories
Primary Antibodies
Cellular Localization
Cytoplasmic
Chromosome Location
17q21.2
Clonality
Monoclonal
Description
This MAb recognizes a protein of 56.5kDa, identified as cytokeratin 10 (CK10). CK10 is expressed in all suprabasal layers of the epidermis. In the epidermis, expression of CK10 strictly parallels the extent of differentiation; it is absent in the basal layer, appears in the first suprabasal layers and increases in concentration towards the granular layer. However, CK10 is rarely detected in early stages of vulvar squamous carcinomas (tumors less than 2 cm, clinical stage I) regardless of the tumor grade. In larger and more advanced tumors (greater than 2 cm, clinical stages II and III), CK10 is detected very frequently. Expression of CK10 is related to maturation of malignant keratinocytes, being preferentially detected in more-differentiated parts.
Host
Mouse
Immunogen
Recombinant human KRT10 protein
Isotype
IgG1 Kappa
Positive Control
A431, HeLa, MCF7 cells. Esophagus or tonsil.
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
P13645 (Human)
Uniprot
99936
Gene Id
3858 (Human)
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
0.5-1μg/ml
IHC-F
0.1-0.2μg/ml for 30 min at RT
Reviews of Anti-Cytokeratin 10 Antibody BHA10202787