CK-7 ; K2C7 ; Keratin 55K Type II Cytoskeletal ; Keratin Simple Epithelial Type 1 K7 ; Keratin Type II Cytoskeletal 7 ; Krt2-7 ; KRT7 ; Sarcolectin ; SCL ; Type II Mesothelial Keratin K7
Categories
Primary Antibodies
Cellular Localization
Cytoplasmic
Chromosome Location
12q13.13
Clonality
Monoclonal
Description
It recognizes an intermediate filament protein (IFP) of 55kDa, which is identified as cytokeratin 7. This MAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This MAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
Host
Mouse
Immunogen
OTN 11, ovarian carcinoma cell line
Isotype
IgG1
Positive Control
HeLa cells. Carcinoma of ovary, lung, cervix, or breast
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
P08729
Uniprot
411501
Gene Id
3855
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
0.5-1μg/ml
IHC-F
0.5-1.0μg/ml for 30 minutes at RT
Reviews of Anti-Cytokeratin 7 (KRT7) Antibody BHA10202642