Bence Jones Protein ; BJP ; IGLC 1/2/3 ; Mcg Marker ; Paraprotein
Categories
Primary Antibodies
Cellular Localization
Cell Surface, Cytoplasmic (Secreted)
Chromosome Location
22q11.22
Clonality
Monoclonal
Description
This MAb is specific to lambda light chain of immunoglobulin and shows no cross-reaction with lambda light chain or any of the five heavy chains. In mammals, the two light chains in an antibody are always identical, with only one type of light chain, kappa or lambda. The ratio of Kappa to Lambda is 70:30. However, with the occurrence of multiple myeloma or other B-cell malignancies this ratio is disturbed. Antibody to the lambda light chain is reportedly useful in the identification of leukemias, plasmacytomas, and certain non-Hodgkin's lymphomas. Demonstration of clonality in lymphoid infiltrates indicates that the infiltrate is malignant.
Host
Mouse
Immunogen
Purified human full-length lambda light chain
Isotype
IgG1 Kappa
Positive Control
293T, Raji or hPBL cells. Tonsil or Spleen
Reactivity
Human
Recombinant
FALSE
Regulatory
RUO
Swissprot
P01701 ; P01842
Uniprot
449585
Gene Id
3535;3546
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, WB, IHC-F
Description
(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
1-2μg/ml
WB
0.5-1μg/ml
IHC-F
0.5-1μg/ml for 30 minutes at RT
Reviews of Anti-Lambda Light Chain Antibody BHA10202323