Heat shock 27kDa protein ; Estrogen-regulated 24kDa protein ; Heat shock 25kDa protein 1 ; Heat shock 28kDa protein 1 ; Heat shock protein beta-1 ; HMN2B ; Hsp25 ; Hsp28 ; HspB1 ; Stress-responsive protein 27 (SRP27)
Categories
Primary Antibodies
Cellular Localization
Cytoplasmic, Nuclear
Chromosome Location
7q11.23
Clonality
Monoclonal
Description
It recognizes a 24-27kDa estrogen-regulated protein, identified as heat shock protein 27 (hsp27). Hsp27 was recently found to be identical to the estrogen-induced �p29� and �24K� protein. About 50% of breast carcinomas are positive for hsp27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells.
Host
Mouse
Immunogen
Recombinant human HSPB1 protein
Isotype
IgG1 Kappa
Positive Control
~50% of Breast Carcinomas are positive for hsp27 especially those that are also positive for ER and/or PgR.
Reactivity
Human, Chimpanzee, Sheep, Rat, Mouse, Chicken
Recombinant
FALSE
Regulatory
RUO
Swissprot
P04792
Uniprot
520973
Gene Id
3315
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA at 1.0mg/ml
Applications
FACS, IF, WB, IHC-F
Description
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
0.5-1μg/ml
WB
0.25-0.5μg/ml
IHC-F
0.5-1μg/ml for 30 minutes at RT
Reviews of Anti-HSP27 (Heat Shock Protein 27) Antibody BHA10201947