Recognizes a protein of 16-27kDa, identified as human interferon-�II) (IFN-(II). Its epitope maps between aa43-53 of the IFN-II) (total aa172). This MAb is specific for IFN-(II) and does not cross-react with IFN-(I). Epitopes of N27 and N39 MAb s are different and represent a good combination of antibodies to set up an ELISA assay for the quantitation of IFN-(II) after viral infections. The IFN- family consists of 24 or more genes or pseudo-genes. IFN-�II) is one of the two distinct families (I and II) of human IFN-. The interferon are mainly produced by lymphocytes, monocytes, macrophages, and cell lines such as Namalwa and KG1 following induction by viruses, nucleic acids, and glucocorticoid hormones. They are involved in virus resistance on target cells, inhibition of cell proliferation, induction of cytokines and regulation of expression of MHC class I antigens.
Host
Mouse
Immunogen
Purified recombinant human IFN-alpha2
Isotype
IgG1 Kappa
Positive Control
Stimulated human peripheral blood mononuclear cells
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA; 0.05% azide. Also available WITHOUT BSA; azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
ELISA, Blocking Activity, FACS, IF
Description
Optimal dilution for a specific application should be determined., ELISA ; Blocking Activity ; FACS ; IF
Dilution
ELISA
For coating, order Ab without BSA
Blocking Activity
Order Ab without Azide
FACS
0.5-1μg/million cells
IF
0.5-1μg/ml
Reviews of Anti-Interferon alpha-2 (IFNA2) Antibody BHA10202059