Anti-HLA-Aw32 & HLA-A25 (MHC I) Antibody BHA10201783

Mfr.No.	3105-MSM4-P0
Alternative Names	HLA-A ; HLA class 1A ; MHC class I HLA-A
Antigen	HLA-Aw32 & HLA-A25 (MHC I)
Categories	Primary Antibodies
Cellular Localization	Cell Surface
Chromosome Location	6q21.3
Clonality	Monoclonal
Conjugate	Unconjugated
Description	This MAb reacts with cells bearing HLA-A25 or HLA-Aw32 antigens. In addition, a reaction was observed with a cell of phenotype A2, Aw31; B17, Bw49. HLA-A, with HLA-B and HLA-C, belongs to major histocompatibility complex (MHC) class I antigens and expresses constitutively on all nucleated cells. HLA system comprises closely linked genes controlling highly polymorphic proteins involved in the presentation of peptides to the T-cell receptor, inhibition of NK cell cytotoxicity, and rejection of tissue allotransplantation. Specific alleles at HLA loci are associated with diseases. This MAb is specifically applicable for typing peripheral T cells for the antigens HLA-A25 and HLA-Aw32.
Host	Mouse
Immunogen	Normal human peripheral blood lymphocytes of phenotype A1, Aw32, B7, B37, Cw-, Cw-, DR2, DRw10
Isotype	IgG2a Kappa
Positive Control	THP-1 and Jurkat cells. Tonsil or lymph node.
Reactivity	Human
Recombinant	FALSE
Regulatory	RUO

Swissprot	P01889; P30443; P30499
Uniprot	181244; 654404; 77961
Gene Id	3105;3106;3107

Buffer	200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration	200µg/ml
Description	There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity	Purified Ab with BSA and Azide at 200μg/ml

Applications

FACS, IF, IHC-F

Description

(Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; IHC-F

Dilution

FACS	0.5-1μg/million cells in 0.1ml
IF	0.5-1μg/ml
IHC-F	0.5-1μg/ml for 30 minutes at RT

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SKU	Supplier No.	Size	Your price	Quantity
BHA10201783	3105-MSM4-P0	20µg	$204.67		Add to Cart
BHA10201783	3105-MSM4-P0	100µg	$393.20		Add to Cart

Anti-HLA-Aw32 & HLA-A25 (MHC I) Antibody BHA10201783

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