DRB1 ; HLA class II histocompatibility antigen, DR-1 beta chain ; HLA-DR-beta 1 ; HLA-DRB1 ; human leucocyte antigen DRB1 ; Leucocyte antigen DR beta 1 chain ; lymphocyte antigen DRB1 ; major histocompatibility complex, class II, DR beta 1 ; MHC class II HLA-DR beta 1 chain ; MHC class II HLA-DR-beta cell surface glycoprotein
Antigen
HLA-DRB (MHC II)
Categories
Primary Antibodies
Cellular Localization
Cell Surface
Chromosome Location
6p21.3
Clonality
Monoclonal
Conjugate
Unconjugated
Description
This MAb reacts with the beta-chain of HLA-DRB1 antigen, a member of MHC class II molecules. It does not cross react with HLA-DP and HLA-DQ. Its epitope is different from that of MAb L243. HLA-DR is a heterodimeric cell surface glycoprotein comprised of a 36kDa alpha (heavy) chain and a 28kDa beta (light) chain. It is expressed on B-cells, activated T-cells, monocytes/macrophages, dendritic cells and other non-professional APCs. In conjunction with the CD3/TCR complex and CD4 molecules, HLA-DR is critical for efficient peptide presentation to CD4+ T cells. It is an excellent histiocytic marker in paraffin sections producing intense cytoplasmic staining. True histiocytic neoplasms are similarly positive. HLA-DR antigens also occur on a variety of epithelial cells and their corresponding neoplastic counterparts. Loss of HLA-DR expression is related to tumor microenvironment and predicts adverse outcome in diffuse large B-cell lymphoma.
Host
Mouse
Immunogen
Activated human peripheral blood mononuclear cells
Isotype
IgG2b Kappa
Positive Control
Ramos, Daudi or HuT78 cells. Tonsil or lymph node
Reactivity
Human, Monkey
Recombinant
FALSE
Regulatory
RUO
Swissprot
P01911
Uniprot
534322
Gene Id
3123
Buffer
200μg/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0mg/ml.
Concentration
1.0mg/ml
Description
There are no warranties, expressed or implied, which extend beyond this description. Company is not liable for any personal injury or economic loss resulting from this product.
Purity
Purified Ab WITHOUT BSA and Azide at 1.0mg/ml
Applications
FACS, IF, WB, IHC-F
Description
(Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes); Optimal dilution for a specific application should be determined., FACS ; IF ; WB ; IHC-F
Dilution
FACS
0.5-1μg/million cells in 0.1ml
IF
0.5-1μg/ml
WB
0.5-1μg/ml
IHC-F
0.25-0.5μg/ml for 30 minutes at RT
Reviews of Anti-HLA-DRB (MHC II) Antibody BHA10201888